CASP3 Antibody

SKU: orb1248971

Description

CASP3 Antibody

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 32kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6kDa according to Human NP_004337.2 and 31.4kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65kDa was also consistently observed in MOLT4 and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1µg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RDVSKEDHSKRS
• Target: CASP3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CASP3, caspase 3, caspase 3, apoptosis-related cysteine protease, HGNC:1504, caspase 3, apoptosis-related cysteine peptidase, CPP32, CPP32B, SCA-1, PARP cleavage protease, SREBP cleavage activity 1, Yama, apopain, cysteine protease CPP32, procaspase3

orb1248971 (1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248971 (0.5 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248971 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI.

Quick Database Links

Gene Symbol

CASP3

UniProt

P42574

RefSeq (Protein):NP_116786.1, NP_004337.2