Cardiac Troponin T Rabbit Polyclonal Antibody

• Catalog Number: orb182936
• Category: Antibodies
• Description: Cardiac Troponin T Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cardiac Troponin T (201-298/298aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 36 kDa
• Target: TNNT2
• UniProt ID: P45379
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cardiac muscle troponin T; Cardiomyopathy dilated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: U-2OS. Primary Antibody (green line): Rabbit Anti-TNNT2 antibody (orb182936), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Cell: Neonatal rat ventricular cardiomyocytes, dilution: 1:400, Incubation: Anti-Cardiac Troponin T Antibody, unconjugated (orb182936), DAPI was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cardiac Troponin T) Polyclonal Antibody, Unconjugated (orb182936) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Positive control: H9C2 cells, Concebtration: 2 µg/10^6 cells, Incubation conditions: Avoid light, 30 minutes on the ice.

Sample: H9C2 (Rat) Cell Lysate at 30 ug, Primary: Anti-alpha smooth muscle Actin (orb182936) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kD, Observed band size: 36 kD.

Tissue/Cell: rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TNNT2 Polyclonal Antibody, Unconjugated (orb182936) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TNNT2 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.