Cardiac Troponin T Rabbit Polyclonal Antibody (PE)

SKU: orb492827

Description

Cardiac Troponin T Rabbit Polyclonal Antibody (PE)

Images & Validation

• Tested Applications: FC, ICC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cardiac Troponin T (201-298/298aa)
• Target: TNNT2
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Cardiac muscle troponin T; Cardiomyopathy dilated 1D(autosomal dominant); Cardiomyopathy hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; MGC3889; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; RCM3; TNNT 2; TNNT2; TNNT2_HUMAN; TnTC; Troponin T cardiac muscle; Troponin T type 2(cardiac); Troponin T type 2 cardiac; Troponin T, cardiac muscle; Troponin T2; Troponin T2 cardiac.

Blank control: U-2OS. Primary Antibody (green line): Rabbit Anti-TNNT2 antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

TNNT2