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Cardiac Troponin T Rabbit Polyclonal Antibody (FITC)

Catalog Number: orb187249

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb187249
CategoryAntibodies
DescriptionCardiac Troponin T Rabbit Polyclonal Antibody (FITC)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IF
Predicted ReactivityBovine, Canine, Porcine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Cardiac Troponin T (201-298/298aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeFlow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500
ConjugationFITC
TargetTNNT2
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCardiac muscle troponin T; Cardiomyopathy dilated
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NoteFor research use only
Expiration Date12 months from date of receipt.
Cardiac Troponin T Rabbit Polyclonal Antibody (FITC)

Blank control: H9C2. Primary Antibody (green line): Rabbit Anti-Desmin antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Cardiac Troponin T Rabbit Polyclonal Antibody (FITC)

Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-Cardiac Troponin T antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Cardiac Troponin T Rabbit Polyclonal Antibody (FITC)

Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-Cardiac Troponin T antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Cardiac Troponin T Rabbit Polyclonal Antibody (FITC)

Cell: Neonatal rat ventricular cardiomyocytes, dilution: 1:200, Incubation: Anti-Cardiac Troponin T Antibody, conjugated (orb187249), DAPI was used to stain the cell nuclei.