You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb187249 |
---|---|
Category | Antibodies |
Description | Cardiac Troponin T Rabbit Polyclonal Antibody (FITC) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF |
Predicted Reactivity | Bovine, Canine, Porcine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human Cardiac Troponin T (201-298/298aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | Flow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500 |
Conjugation | FITC |
Target | TNNT2 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | Cardiac muscle troponin T; Cardiomyopathy dilated Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Blank control: H9C2. Primary Antibody (green line): Rabbit Anti-Desmin antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-Cardiac Troponin T antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-Cardiac Troponin T antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.
Cell: Neonatal rat ventricular cardiomyocytes, dilution: 1:200, Incubation: Anti-Cardiac Troponin T Antibody, conjugated (orb187249), DAPI was used to stain the cell nuclei.
ICC, IF | |
Human | |
Rabbit | |
Polyclonal | |
FITC |
FC | |
Bovine, Canine, Guinea pig, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
FITC |