Cardiac Troponin T Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb187249
• Category: Antibodies
• Description: Cardiac Troponin T Rabbit Polyclonal Antibody (FITC)
• Target: TNNT2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: FITC
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human Cardiac Troponin T (201-298/298aa)
• Tested applications: FC, ICC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Cardiac muscle troponin T; Cardiomyopathy dilated
• Note: For research use only

Blank control: H9C2. Primary Antibody (green line): Rabbit Anti-Desmin antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-Cardiac Troponin T antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-Cardiac Troponin T antibody (orb187249), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Cell: Neonatal rat ventricular cardiomyocytes, dilution: 1:200, Incubation: Anti-Cardiac Troponin T Antibody, conjugated (orb187249), DAPI was used to stain the cell nuclei.