CaMKII (Alpha-Specific) Antibody

• Catalog Number: orb1822494
• Category: Antibodies
• Description: Mouse Anti-Rat CaMKII (Alpha-Specific) Monoclonal IgG1
• Target: CaMKII
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Bovine, Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein G Purified
• Immunogen: Partially purified rat CaMKII
• UniProt ID: P11798
• MW: 50-60kDa
• Tested applications: ELISA, ICC, IF, IHC, IP, RIA, WB
• Dilution range: WB (1:10000), IHC (1:2000), ICC/IF (1:50); optimal dilutions for assays should be determined by the user.
• Application notes: 0.1 µg/ml was sufficient for detection of CamKII in 20 µg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
• Clone Number: 6G9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CamK2 Antibody, CamK2A Antibody, CamK2B Antibody,
• Research Area: Protein Biochemistry, Signal Transduction
• Note: For research use only
• Entrez: 12322
• NCBI: NP_033922.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.

Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000.

Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X.

Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Magnification: 40x.