BUB3 Antibody

• Catalog Number: orb1240078
• Category: Antibodies
• Description: BUB3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Bub3 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3.The immunogen is located within the last 50 amino acids of Bub3.
• Concentration: 1 mg/mL
• Dilution range: Bub3 antibody can be used for detection of bub3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: BUB3
• UniProt ID: O43684
• NCBI: O43684
• Storage: Bub3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Bub3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Bub3 Antibody: BUB3L, hBUB3, Mitotic checkpoint pr
• Note: For research use only
• Application notes: Bub3 antibody can be used for detection of bub3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 µg/mL.

Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 µg/mL.

Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 µg/mL.