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| Catalog Number | orb76159 |
|---|---|
| Category | Antibodies |
| Description | Bub3 Antibody |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Purification | Immunogen affinity purified. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Bub3, identical to the related rat and mouse sequences. |
| UniProt ID | O43684 |
| MW | 37 kDa |
| Tested applications | ICC, IHC, IHC-Fr, WB |
| Application notes | Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, MouseImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, MouseWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Cross Reactivity | No cross-reactivity with other proteins |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Mitotic checkpoint protein BUB3; BUB3 |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |


Confocal immunofluoroscence analysis of HELA Cell using BUB3 antibody

Western blot analysis of Lane 1: HELA Cell Lysate, Lane 2: A549 Cell Lysate, Lane 3: JURKAT cell Lysate, Lane 4: COLO320 cell Lysate using BUB3 antibody

Immunohistochemical analysis of

ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
IF, IHC, WB | |
Bovine, Equine, Guinea pig, Rabbit, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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