BrdU Antibody

SKU: orb345681

Description

BrdU antibody

Images & Validation

• Tested Applications: DOT, ELISA, FC, IF, IHC
• Dilution Range: ELISA: 1:2000 - 1:10,000, FC: 1:50-1:100, IHC: 1:100-1:500, IF: 1:500
• Reactivity: Other
• Application Notes:
  Anti-BrdU Antibody has been tested by ELISA, slot blot, IHC, and is suitable for immunofluorescence microscopy and flow cytometry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated BrdU thymidine analog from replicated cells

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.
• Purity: BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store BrdU Antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-BrdU antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine