BrdU Antibody

• Catalog Number: orb154393
• Category: Antibodies
• Description: Mouse monoclonal antibody to BrdU
• Target: BrdU
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Bromodeoxyuridine conjugated to BSA
• Tested applications: FC, IHC-Fr, IHC-P
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
• Antibody Type: Primary Antibody
• Clone Number: Bu20a
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 5-bromodeoxyuridine
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry intracellular staining pattern of BrdU incorporated K562 cells stained using anti-BrdU (Bu20a) purified antibody (concentration in sample 4 μg/ml, GAM FITC).

Flow cytometry multicolor intracellular staining pattern of BrdU incorporated K562 cellular suspension using anti-BrdU (Bu20a) purified antibody (concentration in sample 4 μg/ml, GAM FITC) and 7-AAD.

Separation of human BrdU positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (intracellular staining) of BrdU incorporated K562 cells stained using anti-BrdU (Bu20a) purified antibody (concentration in sample 4 μg/ml, GAM FITC).