BrdU Antibody (Biotin)

SKU: orb3123919

Description

BrdU Antibody (Biotin)

Images & Validation

• Tested Applications: FC, IHC-Fr, IHC-P
• Application Notes:
  Flow cytometry: Recommended dilution: 1-5 μg/ml.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: Bu20a
• Immunogen: Bromodeoxyuridine conjugated to BSA
• Target: BrdU
• Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin

Storage & Handling

• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5-bromodeoxyuridine

Flow cytometry intracellular staining pattern of BrdU incorporated K562 cells stained using anti-BrdU (Bu20a) Biotin antibody (concentration in sample 0.5 μg/ml, Streptavidin APC).

Separation of cells stained using anti-BrdU (Bu20a) Biotin antibody (concentration in sample 0.5 μg/ml, Streptavidin APC, red-filled) from cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis (intracellular staining) of BrdU incorporated K562 cell suspension.

Flow cytometry multicolor intracellular staining pattern of BrdU incorporated K562 cellular suspension using anti-BrdU (Bu20a) Biotin antibody (concentration in sample 0.5 μg/ml, Streptavidin APC) and Propidium Iodide.

Quick Database Links

Gene Symbol

BrdU

NCBI Gene ID

35