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BrdU Antibody

SKU: orb345680

Description

BrdU antibody

Images & Validation

Tested ApplicationsDOT, ELISA, FC, IF, IHC
Dilution RangeELISA: 1:2000 - 1:10,000, FC: 1:50-1:100, IHC: 1:100-1:500, IF: 1:500
ReactivityOther
Application Notes
Anti-BrdU Antibody has been tested by ELISA, slot blot, IHC, and is suitable for immunofluorescence microscopy and flow cytometry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated BrdU thymidine analog from replicated cells

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.
PurityAnti-BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageStore BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLiquid (sterile filtered)
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.23 mg/mL
Expiration Date12 months from date of receipt.
Dry Ice ShippingPlease note: This product requires shipment on dry ice. A dry ice surcharge will apply.
DisclaimerFor research use only

Alternative Names

rabbit anti-BrdU Antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
DOT
Dot Blot
View Protocol

Available Sizes

Select a size below

100 μg
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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