Brain-derived neurotrophic factor (BDNF) Antibody

SKU: orb3167679

Description

Our Anti-Brain-derived neurotrophic factor (BDNF) rabbit polyclonal primary antibody detects human and other mammals (predicted) Brain-derived neurotrophic factor (BDNF), and is affinity-purified. It is validated for use in ELISA.

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: WB: 0.2-1 µg/mL
• Reactivity: Human
• Predicted Reactivity: Mammal
• Application Notes:
  WB: Antibody detects 14 kDa mature BDNF monomer and 32 kDa proBDNF monomer in cell lysate and tissue homonenate. Antibody has only been tested on cell lysate and tissue homogenate of human origin. Acid-treated samples may give cleaner blots, and enhance signals for BDNF. orb3167679 is not recommended for human serum samples. For human serum analysis, we recommend mouse monoclonal antibody to rhBDNF (orb3167453), or rabbit polyclonal antibody to BDNF peptide 1-10 (orb3167552, whole serum; orb3167541, IgG). Flow Cytometry: ~2 µg per 10^6 cells, methanol fixation. Note: orb3167679 cannot be used to distinguish the flow cytometry signal originating from mature BDNF versus proBDNF.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antibody was raised against a GST-tagged rhBDNF fusion protein and expressed in and purified from E. coli.
• Target: Brain-derived neurotrophic factor (BDNF)
• Molecular Weight: 14 kDa, 32 kDa (See application details.)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from a solution containing PBS pH 7.4, 3% trehalose, with 0.05% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-derived neurotrophic factor; Abrineurin

Quick Database Links

Gene Symbol

Brain-derived neurotrophic factor (BDNF)

UniProt

P23560