BDNF Rabbit pAb Discontinued

SKU: orb10181

Description

BDNF antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg /test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Rabbit, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BDNF (81-180/247 aa)
• Target: BDNF
• Molecular Weight: 13/27 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Abrineurin; Brain Derived Neurotrophic Factor; MGC34632; Neurotrophin; BDNF_HUMAN.

Blank control: HepG2 (blue). Primary Antibody: Rabbit Anti-BDNF antibody (orb10181, Green), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (orb10181, 1 µg/1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Brain (Rat) Lysate at 30 ug, Heart (Rat) Lysates, 30 ug, Primary: Anti-BDNF (orb10181) at 1:200, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 3000, Predicted band size: 13kD, 25kD, Observed band size: 25kD.

Tissue/cell: rabbit brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated (orb10181) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

BDNF

UniProt

P23560

Marjan Mirsalehi # 1, Alimohamad Asghari # 1, Masoud Nasiri Nasab Rafsanjani 1, Akram Pourbakht 2, Maryam Balali 1, Sayedali Ahmadi 3, Zeinab Akbarnejad 4 Developmental timing of auditory deprivation influences spatial memory and hippocampal plasticity in rats Sci Rep, (2025)

PubMed: 41315376

Applications

WB

Reactivity

Rat