BDNF Antibody (C-term)

SKU: orb1928713

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: IF - 1:10-50, WB - 1:1000, FC - 1:10-50
• Reactivity: Human
• Predicted Reactivity: Equine, Mouse, Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB14839
• Immunogen: This BDNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the C-terminal region of human BDNF. Antigen Region: 206-236 aa.
• Target: BDNF {ECO:0000303|PubMed:28397838, ECO:0000312|HGNC:HGNC:1033}
• Molecular Weight: 27818 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-derived neurotrophic factor, BDNF, Abrineurin, BDNF

Confocal immunofluorescent analysis of BDNF Antibody (C-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of CEM cells using BDNF Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain reacted with BDNF Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-BDNF Antibody (C-term) at 1:1000 dilution. Lane 1: human cerebellum lysate. Lane 2: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

BDNF {ECO:0000303|PubMed:28397838, ECO:0000312|HGNC:HGNC:1033}

UniProt

P23560

RefSeq (Protein):NP_001137277.1, NP_001137282.1, NP_733927.1, NP_733928.1, NP_001137288.1, NP_001700.2, NP_001137279.1, NP_001137280.1, NP_001137284.1, NP_001137285.1, NP_001137278.1, NP_001137281.1, NP_001137286.1, NP_001137283.1