Brain-derived neurotrophic factor (BDNF) Antibody

SKU: orb3167555

Description

Our Anti-Brain-derived neurotrophic factor (BDNF) rabbit polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Brain-derived neurotrophic factor (BDNF), and is whole serum. It is validated for use in IHC-Frozen, Neutralization, WB.

Images & Validation

• Tested Applications: IHC, NeA, WB
• Dilution Range: WB: 1:1000; IHC: 1:1000; ELISA: 1:1000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mammal
• Application Notes:
  Inhibition of biological activity in vitro/in vivo: 1:10 to 1:50 for inhibition of biological activity in vitro. Use neat for in vivo studies at 5-10 uL/g body weight. This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western Blotting: Antibody does detect BDNF forms in tissue lysates but there are multiple bands present, many of which are uncharacterized. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform (see blot examples). In cell lysates, only 18 kDa and 32 kDa BDNF are detected. The reason for these differences has not been characterized. Alternative antibodies for Western Blotting are: R-017-500 (IgG-purified form of orb3167555 for tissue homogenate analysis); orb3167679 (cell lysates and tissue homogenates), orb3167552/orb3167541 (cell lysates, tissue homogenates and human serum); orb3167453/orb3167452 (human serum and tissue homogenates).

  Read more...

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Mixed
• Immunogen: Recombinant human BDNF
• Target: Brain-derived neurotrophic factor (BDNF)
• Molecular Weight: 14, 18, 32 kDa (see application details)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-derived neurotrophic factor; Abrineurin; proBDNF;

Quick Database Links

Gene Symbol

Brain-derived neurotrophic factor (BDNF)

UniProt

P23560