Brain-derived neurotrophic factor (BDNF) Antibody

SKU: orb3167541

Description

Our Anti-Brain-derived neurotrophic factor (BDNF) rabbit polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Brain-derived neurotrophic factor (BDNF), and is IgG. It is validated for use in ELISA, IHC-Frozen, WB.

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: WB: 1-10 µg/mL; IHC: 1-10 µg/mL; ELISA: 1-10 µg/mL
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mammal
• Application Notes:
  WB: In Western Blotting, this antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). IHC: Antibody works well in immunohistochemistry with the proper fixation, pretreatments and dilution. Formal fixed, paraffin embedded tissue is not recommend. Recommended fixation is Zamboni fixative or light 4% PFA fixation on fixed, frozen tissue. Recommended dilution is 1-10 µg/mL for immunohistochemistry at 4 degrees centigrade for 2-48 hours. ELISA: 1-10 µg/mL capture/detection.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst mammalian species.
• Target: Brain-derived neurotrophic factor (BDNF)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from PBS, pH 7.4, without preservatives.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-derived neurotrophic factor; Abrineurin; proBDNF;

Quick Database Links

Gene Symbol

Brain-derived neurotrophic factor (BDNF)

UniProt

P23560