Brain-derived neurotrophic factor (BDNF) Antibody

SKU: orb3167534

Description

Our Anti-Brain-derived neurotrophic factor (BDNF) rabbit polyclonal primary antibody detects human, mouse, other mammals (predicted), and rat Brain-derived neurotrophic factor (BDNF), and is IgG. It is validated for use in ELISA, IHC-Frozen, Neutralization, WB.

Images & Validation

• Tested Applications: ELISA, IHC, NeA, WB
• Dilution Range: WB: 1-10 µg/mL; IHC: 1-10 µg/mL; ELISA: 0.1 µg/mL
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mammal
• Application Notes:
  Can be used for inhibition of biological activity in vitro/in vivo: in vitro 1-10 µg/mL, use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum stains cell bodies and some nerve terminals in the dorsal horn of the rat spinal cord, however, does not stain finest nerve terminals. Western blot: Tissue homogenate is a recommended sample application for Western blotting. The antibody detects 14 kDa (mature BDNF), 32 kDa (proBDNF) and a 18 kDa BDNF isoform, however numerous other non-characterized bands may also be present. In cell lysates, only 18 kDa and 32 kDa BDNF are detected. Alternative antibodies for Western blotting are: R-1707-100 (cell lysates and tissue homogenates), R-083-100/R-066-500 (cell lysates, tissue homogenates and human serum); M-1744-50/100 (human serum and tissue homogenates).

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant human BDNF
• Target: Brain-derived neurotrophic factor (BDNF)
• Molecular Weight: 14, 18, 32 kDa (see application details)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from a solution containing PBS pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-derived neurotrophic factor; Abrineurin; proBDNF;

Quick Database Links

Gene Symbol

Brain-derived neurotrophic factor (BDNF)

UniProt

P23560