Brain-derived neurotrophic factor (BDNF) Antibody

SKU: orb3167453

Description

Our Anti-Brain-derived neurotrophic factor (BDNF) mouse monoclonal primary antibody detects human, mouse, other mammals (predicted), and rat Brain-derived neurotrophic factor (BDNF), and is IgG. It is validated for use in ELISA, WB.

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 0.2-2 µg/mL; IHC: 1-4 µg/mL; ICC: 1-4 µg/mL; ELISA: 50-100 ng/mL
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mammal
• Application Notes:
  M-1744-100 antibody detects 14 kDa mature BDNF monomer in human serum human and acid treated human and rodent whole tissue homogenates. In simple Tris-homogenate human brain lysates, M-1744-100 detects mature BDNF at 14 kDa as well as an uncharacterized band at ~50 kDa. No proBDNF band is detected in our samples, although M-1744-100 will detect proBDNF in purified form on blots. Additionally, M-1744-100 detects an additional band 18 kDa BDNF isoform human serum. For cell lysates, some caution is advised as in our hands M-1744-100 fails to detect mature BDNF on SH-SY5Y or C6 cell lysates but does detect mature BDNF in neuronal cell culture lysates. The reason for these differences has not been characterized. For detection of mature BDNF in cell lysates such as SH-SY5Y we recommend affinity-purified rabbit polyclonal antibody to rhBDNF (R-1707-100), or rabbit polyclonal antibody to BDNF peptide 1-10 (R-083-100, whole serum; R-066-500, IgG). IF: 4% formaldehyde fixation and permeabilization. Staining can be weak until optimized. M-1744-100 is not recommended for Flow Cytometry at this time.

  Read more...

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 4C8
• Immunogen: Recombinant human mature BDNF expressed in E.coli
• Target: Brain-derived neurotrophic factor (BDNF)
• Molecular Weight: 14 kDa (see application notes)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from a solution containing PBS pH 7.4, 0.1% trehalose, with 0.1% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Brain-derived neurotrophic factor (BDNF)

UniProt

P23560