Biotin Antibody Fluorescein Conjugated

SKU: orb345830

Description

Biotin antibody (FITC)

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF: 1:500-1:2,500
• Application Notes:
  Biotin Antibody Fluorescein Conjugated has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Purity: Biotin Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Biotin Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Biotin Antibody

Western Blot of Rabbit anti-Biotin Fluorescein Conjugated Antibody. Lane 1: Biotin Conjugated Guinea Pig IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Guinea Pig IgG. Other band(s): None.