Mouse FITC, conjugated with Biotin Antibody

• Catalog Number: orb21175
• Category: Antibodies
• Description: Biotin-conjugated purified monoclonal mouse antibody to fluorescein isothiocyanate isomer 1. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Form/Appearance: Biotin conjugated purified mouse IgG1 lyophilized from a solution in phosphate buffered saline (pH 7.2).
• RRID: AB_10751436
• Antibody Type: Primary Antibody
• Clone Number: NI 239
• Source: Immunogen: Highly purified fluorescein isothiocyanate isomer 1.
• Storage: The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
• Note: For research use only