Mouse anti FITC Antibody

SKU: orb21166

Description

Monoclonal mouse antibody to fluorescein isothiocyanate isomer 1. To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: NI 239
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Delipidated, heat inactivated, lyophilized, stable whole ascites. IgG concentration in the product is 1.0 mg/ml. No foreign proteins added.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only