beta Tubulin

SKU: orb1787839

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB: 1:4000, FC: 1:25
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: C. elegans, Drosophila, Hamster, Other, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human. Antigen Region: 46-78 aa.
• Target: Tubb5
• Molecular Weight: 49671 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tubulin beta-5 chain, Tubb5

All lanes: Anti-beta Tubulin Antibody at 1:4000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: C2C12 whole cell lysate. Lane 7: PC-12 whole cell lysate. Lane 8: C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing NIH/3T3 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Quick Database Links

Gene Symbol

Tubb5

UniProt

P99024