beta Tubulin antibody

SKU: orb555945

Description

Rabbit polyclonal antibody to beta Tubulin

Images & Validation

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Item 1 of 9

Tested Applications	ICC, IHC, IHC-P, WB
Dilution range	WB: 1:500-1:20000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
Reactivity	Hamster, Human, Mouse, Rat, Zebrafish
Application Notes	Positive control: NIH-3T3 , 293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.
Target	beta Tubulin
Purity	Purified by antigen-affinity chromatography.
Purification	Purified by antigen-affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
Form/Appearance	Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Buffer/Preservatives	0.025% ProClin 300. 1XPBS pH 7, 20% Glycerol
Concentration	1 mg/ml
Disclaimer	For research use only

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Immunohistochemical staining of human breast cancer using beta Tubulin antibody

Immunohistochemical staining of AGS xenograft using beta Tubulin antibody

Immunohistochemical staining of rat spinal cord using beta Tubulin antibody

Immunohistochemical staining of rat spleen using beta Tubulin antibody

Western blot analysis of Various whole cell using beta Tubulin antibody

Western blot analysis of NIH-3T3 cells using beta Tubulin antibody

Immunofluorescence analysis of HeLa cells using beta Tubulin antibody

Quick Database Links

Gene Symbol

beta Tubulin

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ICC

Immunocytochemistry

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ICC (1)

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Rat (1)

SM Jalalian Hosseini, J Baharara Combined Effect of Decellularized Rat Brain Tissue and Nerve Growth Factor on 5 Neural Differentiation of PC12 Cell Line in 2D Cell Culture. Pakistan Journal of, (2025)

PubMed:

Seyed Majid Jalalian Hosseini 1 , Javad Baharara2 *, Shahrbanoo Oryan3 and Mohammad Amin Kerachian4 Combined Effect of Decellularized Rat Brain Tissue and Nerve Growth Factor on 5 Neural Differentiation of PC12 Cell Line in 2D Cell Culture Pakistan Journal of Zoology, (2024)

PubMed:

Applications

ICC

Reactivity

Rat