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Catalog Number | orb555945 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to beta Tubulin |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ICC, IHC, IHC-P, WB |
Reactivity | Hamster, Human, Mouse, Rat, Zebrafish |
Isotype | IgG |
Immunogen | Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/ml |
Dilution range | WB: 1:500-1:20000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
Form/Appearance | Liquid: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
Purity | Purified by antigen-affinity chromatography. |
Conjugation | Unconjugated |
Target | beta Tubulin |
Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
Buffer/Preservatives | 0.025% ProClin 300. 1XPBS pH 7, 20% Glycerol |
Note | For research use only |
Application notes | Positive control: NIH-3T3 , 293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 |
Expiration Date | 12 months from date of receipt. |
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Seyed Majid Jalalian Hosseini 1 , Javad Baharara2 *, Shahrbanoo Oryan3 and Mohammad Amin Kerachian4 Combined Effect of Decellularized Rat Brain Tissue and Nerve Growth Factor on 5 Neural Differentiation of PC12 Cell Line in 2D Cell Culture Pakistan Journal of Zoology, (2024)
Applications
Reactivity
Immunohistochemical staining of human breast cancer using beta Tubulin antibody
Immunohistochemical staining of AGS xenograft using beta Tubulin antibody
Immunohistochemical staining of rat spinal cord using beta Tubulin antibody
Immunohistochemical staining of rat spleen using beta Tubulin antibody
Western blot analysis of Various whole cell using beta Tubulin antibody
Western blot analysis of Various whole cell using beta Tubulin antibody
Western blot analysis of Various whole cell using beta Tubulin antibody
Western blot analysis of NIH-3T3 cells using beta Tubulin antibody
Immunofluorescence analysis of HeLa cells using beta Tubulin antibody
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Canine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |