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Description
Images & Validation
−| Tested Applications | ELISA, FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Tubulin-Beta (31-130/444 aa) |
| Target | Beta Tubulin |
| Molecular Weight | 50 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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UniProt Details
−Protocol Information
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Ayantika Kundu 1, Pratiti Ghosh 2, Biswadev Bishayi Verapamil and tangeretin enhances the M1 macrophages to M2 type in lipopolysaccharide-treated mice and inhibits the P-glycoprotein expression by downregulating STAT1/STAT3 and upregulating SOCS3 Int Immunopharmacol, (2024)
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Reactivity
Rituparna Ghosh 1, Biswadev Bishayi 2 Neutralization of TLR2 in combination with either TNF-α or IL-1β antibody reduces the severity of septic arthritis through STAT3/mTOR signalling in lymphocytes Cell Immunol, (2024)
Applications
Reactivity
Ghosh, Rituparna et al. Simultaneous neutralization of TGF-? and IL-6 attenuates Staphylococcus aureus-induced arthritic inflammation through differential modulation of splenic and synovial macrophages Scand J Immunol, e13252 (2023)
Rituparna Ghosh 1, Biswadev Bishayi 2 Endogenous blocking of TLR2 along with TNF-α and IL-1β ameliorates the severity of the S. aureus arthritis via modulating STAT3/SOCS3 expressions in tissue resident macrophages Microb Pathog ., (2024)
Applications
Reactivity
Sharmistha Samanta , Gopinath Mukherjee , Sreya Chattopadhyay , Biswadev Bishayi * Concomitant inhibition of TLR4 and NLRP3 inflammasome in macrophages regulates lipopolysaccharide induced inflammation The Microbe, (2025)
Applications
Reactivity
Gopinath Mukherjee et al. TREATMENT OF MICE WITH TLR4 AND IFN-γ ANTIBODY AND EXOGENOUS IL-10 MODULATES BCL2 AND LC3 EXPRESSION IN RANKL/M-CSF PLUS LPS STIMULATED BONE MARROW MACROPHAGES-LEADING TO BONE RESORPTION researchgate.net, (2025)
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