Beta Tubulin Rabbit pAb Discontinued

SKU: orb11537

Description

beta Tubulin antibody

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, Flow-Cyt=1μg/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Tubulin-Beta (31-130/444 aa)
• Target: Beta Tubulin
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Beta Tubulin (orb11537) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-Beta Tubulin (orb11537) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Sample: Lane 1: Cerebellum (Rat) Lysate at 40 ug, Lane 2: Cerebellum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Ovary (Mouse) Lysate at 40 ug, Lane 6: Heart (Mouse) Lysate at 40 ug, Lane 7: Heart (Rat) Lysate at 40 ug, Primary: Anti-beta Tubulin (orb11537) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

Beta Tubulin

UniProt

P07437

Ayantika Kundu 1, Pratiti Ghosh 2, Biswadev Bishayi Verapamil and tangeretin enhances the M1 macrophages to M2 type in lipopolysaccharide-treated mice and inhibits the P-glycoprotein expression by downregulating STAT1/STAT3 and upregulating SOCS3 Int Immunopharmacol, (2024)

PubMed: 38678669

Applications

WB

Reactivity

Mouse

Rituparna Ghosh 1, Biswadev Bishayi 2 Neutralization of TLR2 in combination with either TNF-α or IL-1β antibody reduces the severity of septic arthritis through STAT3/mTOR signalling in lymphocytes Cell Immunol, (2024)

PubMed: 39312873

Applications

WB

Reactivity

Mouse

Ghosh, Rituparna et al. Simultaneous neutralization of TGF-? and IL-6 attenuates Staphylococcus aureus-induced arthritic inflammation through differential modulation of splenic and synovial macrophages Scand J Immunol, e13252 (2023)

PubMed: 36597222

Rituparna Ghosh 1, Biswadev Bishayi 2 Endogenous blocking of TLR2 along with TNF-α and IL-1β ameliorates the severity of the S. aureus arthritis via modulating STAT3/SOCS3 expressions in tissue resident macrophages Microb Pathog ., (2024)

PubMed: 38160988

Applications

WB

Reactivity

Mouse

Sharmistha Samanta , Gopinath Mukherjee , Sreya Chattopadhyay , Biswadev Bishayi * Concomitant inhibition of TLR4 and NLRP3 inflammasome in macrophages regulates lipopolysaccharide induced inflammation The Microbe, (2025)

PubMed:

Applications

WB

Reactivity

Mouse

Gopinath Mukherjee et al. TREATMENT OF MICE WITH TLR4 AND IFN-γ ANTIBODY AND EXOGENOUS IL-10 MODULATES BCL2 AND LC3 EXPRESSION IN RANKL/M-CSF PLUS LPS STIMULATED BONE MARROW MACROPHAGES-LEADING TO BONE RESORPTION researchgate.net, (2025)

PubMed:

Applications

WB

Reactivity

Mouse