B2M Antibody

SKU: orb750143

Description

Beta-2 Microglobulin/B2M is a component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [UniProt]

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Titering of the B2M antibody may be required for optimal performance.> 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: MGBP2-1
• Immunogen: Full length recombinant human protein was used as the immunogen for the B2M antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human renal carcinoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

IHC testing of FFPE human bladder carcinoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

IHC testing of FFPE human melanoma with B2M antibody (clone MGBP2-1). FFPE testing requires sections to be boiled inpH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

Immunofluorescent staining of human HeLa cells with B2M antibody (green, clone MGBP2-1) and Reddot nuclear stain (red).

Flow cytometry testing of human HeLa cells with B2M antibody (clone MGBP2-1); Red = isotype control, Blue = B2M antibody.

Western blot testing of human Raji cell lysate with B2M antibody (clone MGBP2-1).

Quick Database Links

UniProt

P61769