B2M Antibody

SKU: orb153541

Description

Mouse monoclonal antibody to B2M

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: 3G5H8
• Immunogen: Purified recombinant fragment of human B2M (AA: 21-100) expressed in E. Coli.
• Target: B2M
• Molecular Weight: 13.7kDa

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide.
• Concentration: Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration.
• Disclaimer: For research use only

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of B2M antibody

Flow cytometric analysis of A431 cells lysates using B2M antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded ovarian cancer tissues using B2M antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded esophageal cancer tissues using B2M antibody

Western blot analysis of human B2M using B2M antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using B2M antibody

Western blot analysis of Hela (Lane 1), HEK293 (Lane 2), HepG2 (Lane 3),RAJI (Lane 4), A431 (Lane 5) Jurkat (Lane 6) cell lysate using B2M antibody

Quick Database Links

Gene Symbol

B2M

UniProt

P61769

NCBI Gene ID

567