Bcl-6 Rabbit Polyclonal Antibody

• Catalog Number: orb4674
• Category: Antibodies
• Description: Bcl-6 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Bcl-6 (331-430/706aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 78 kDa
• Target: BCL6
• UniProt ID: P41182
• RRID: AB_10919098
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Bcl-6/5; Bcl-6+Bcl-5; B-cell lymphoma 6 protein is
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human RAMOS cell lysates, Lane 2: Human Raji cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-Bcl-6 (orb4674) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 100 kDa.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Bcl-6) Polyclonal Antibody, Unconjugated (orb4674) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Bcl-6) Polyclonal Antibody, Unconjugated (orb4674) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.