BCORL1 Antibody (N-term)

• Catalog Number: orb1938653
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB28042
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500, WB: 1:500, WB: 1:1000, WB: 1:500, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 190561 Da
• Target: This BCORL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the N-terminal region of human BCORL1.
• UniProt ID: Q5H9F3
• NCBI: NP_068765.3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: BCL-6 corepressor-like protein 1, BCoR-L1, BCoR-li
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-BCORL1 Antibody (N-term) at 1:1000 dilution + Caco2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 183 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-BCORL1 Antibody (N-term) at 1:500 dilution + HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 183 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-BCORL1 Antibody (N-term) at 1:500 dilution + Rat skeletal muscle lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 183 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-BCORL1 Antibody (N-term) at 1:500 dilution + Rat skeletal muscle lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 183 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

BCORL1 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

BCORL1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCORL1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

BCORL1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the BCORL1 antibody detected the BCORL1 protein (arrow).