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Catalog Number | orb1929329 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19040 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:500, IHC-P: 1:50~100, IHC: 1:50, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 192189 Da |
Target | This BCOR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1107-1137 amino acids from the Central region of human BCOR. |
UniProt ID | Q6W2J9 |
NCBI | NP_001116856.1, NP_060215.4, NP_001116855.1, NP_001116857.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | BCL-6 corepressor, BCoR, BCOR, KIAA1575 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
BCOR Antibody (Center S1122) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of paraffin-embedded Human Breast cancer section using Pink1. Diluted at 1: 50 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
BCOR Antibody (Center S1122) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCOR Antibody (Center S1122) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of BCOR Antibody (Center S1122) in 293 cell line lysates (35 ug/lane). BCOR (arrow) was detected using the purified Pab.
Anti-BCOR Antibody (Center S1122) at 1:500 dilution + Mouse liver whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 192 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.