BBC3/PUMA Antibody

SKU: orb1535758

Description

BBC3/PUMA Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, ICC, IF, IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml)
• Reactivity: Human, Mouse
• Application Notes:
  Further information: PUMA antibody can be used for detection of PUMA by Western blot at 2 ug/mL. Antibody can also be used for immunocytochemstry at 1 ug/mL. For immunofluorescence start at 2 ug/mL. Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 23 kDa

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to 14 amino acids near the C-Terminus of human PUMA-a. The sequence is identical between a and b forms of the PUMA proteins. The immunogen is located within the last 50 amino acids of PUMA.
• Target: BBC3 / PUMA
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA, PUMA alpha

Immunofluorescence of PUMA in K562 cells with PUMA antibody at 2 ug/ml.

Quick Database Links

Gene Symbol

BBC3 / PUMA