BBC3/PUMA Rabbit Polyclonal Antibody

• Catalog Number: orb10176
• Category: Antibodies
• Description: BBC3/PUMA Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human BBC3 (131-180/193aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 21 kDa
• Target: BBC3
• UniProt ID: Q9BXH1
• RRID: AB_10751532
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: PUMA; bcl-2 binding component 3; BBC 3; BBC3; BCL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: K562 Cell Lysate at 30 ug, Primary: Anti-BBC3 (orb10176) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kD, Observed band size: 25 kD.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated (orb10176) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.