Puma BH3 Domain Antibody

• Catalog Number: orb1936871
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Puma BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from human Puma BH3 Domain.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q9BXH1
• MW: 20532 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB01353
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Bcl-2-binding component 3, JFY-1, p53 up-regulated
• Note: For research use only
• NCBI: NP_001120714.1, NP_001120712.1, NP_001120713.1, NP_055232.1

Confocal immunofluorescent analysis of Puma BH3 Domain Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Puma BH3 Domain antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Puma BH3 Domain Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of anti-Puma BH3 domain Pab in HL-60 cell lysate. Puma BH3 domain (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.