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BANF1 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IF, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-BANF1 antibody (orb1240141) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human BANF1. The immunogen is located within the last 50 amino acids of BANF1. |
| Target | BANF1 |
| Molecular Weight | Predicted: 10kDObserved: 12 kD Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE. |
| Purification | BANF1 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | BANF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−BANF1 Antibody [orb1239255]
ELISA, IF, WB
Bovine
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
0.1 mg, 0.02 mgBANF1 Recombinant Rabbit Monoclonal Antibody [orb704500]
ELISA, ICC, IF, IHC-Fr, IHC-P
Mouse, Rat
Human, Mouse, Rat
Rabbit
Recombinant
Unconjugated
50 μl, 100 μl

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Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: BANF1 orb1240141 (2 µg/mL), BANF1 orb1239255 (2 µg/mL) and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Kidney Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: BANF1 orb1240141 (A: 0.5 µg/mL and B: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Kidney Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: BANF1 orb1240141 (A: 0.5 µg/mL and B: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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BANF1 Antibody (orb1240141)
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