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Catalog Number | orb704500 |
---|---|
Category | Antibodies |
Description | BANF1 Recombinant Rabbit Monoclonal Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | 11H3 |
Tested applications | ELISA, ICC, IF, IHC-Fr, IHC-P |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant human BANF1 Full length |
Antibody Type | Recombinant Antibody |
Concentration | 1mg/ml |
Dilution range | IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-100, IF=1:100-500, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 10 kDa |
Target | BANF1 |
UniProt ID | O75531 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | BAF; BAF_HUMAN; BANF 1; BANF1; Barrier to autointe Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BANF1) Monoclonal Antibody, Unconjugated (orb704500) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BANF1) Monoclonal Antibody, Unconjugated (orb704500) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (BANF1) Monoclonal Antibody, Unconjugated (orb704500) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.