BANF1 Antibody

SKU: orb1239255

Description

BANF1 Antibody

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-BANF1 antibody (orb1239255) was raised against a peptide corresponding to 19 amino acids near the amino terminus of human BANF1. The immunogen is located within the first 50 amino acids of BANF1.
• Target: BANF1
• Molecular Weight: Predicted: 10kDObserved: 12 kD Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.
• Purification: BANF1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BANF1 Antibody: BAF, NGPS, BCRP1, D14S1460, BAF, BCRG1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Lines. Loading: 15 µg of lysates per lane. Antibodies: BANF1, orb1240141 (2 µg/mL), BANF1, orb1239255 (2 µg/mL) and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: BANF1 orb1239255 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: BANF1, orb1239255 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: BANF1, orb1239255 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of BANF1 in Human Kideny Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with orb1239255 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Quick Database Links

Gene Symbol

BANF1

UniProt

O75531

RefSeq:AAH05942