BANF1 Antibody

• Catalog Number: orb1239255
• Category: Antibodies
• Description: BANF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-BANF1 antibody (orb1239255) was raised against a peptide corresponding to 19 amino acids near the amino terminus of human BANF1. The immunogen is located within the first 50 amino acids of BANF1.
• Concentration: 1 mg/mL
• Dilution range: WB: 1-2 μg/mL; IF: 20 μg/mL.Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 10kDObserved: 12 kD Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.
• Target: BANF1
• UniProt ID: O75531
• NCBI: AAH05942
• Storage: BANF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: BANF1 Antibody: BAF, NGPS, BCRP1, D14S1460, BAF, B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Lines. Loading: 15 µg of lysates per lane. Antibodies: BANF1, orb1240141 (2 µg/mL), BANF1, orb1239255 (2 µg/mL) and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: BANF1 orb1239255 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: BANF1, orb1239255 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: BANF1, orb1239255 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of BANF1 in Human Kideny Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling BANF1 with orb1239255 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).