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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hBAFF (human BAFF). |
| Target | TNFSF13B |
| Purification | Anti-hBAFF specific antibody was purified by affinity chromatography employing immobilized hBAFF matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−TNFSF13B Antibody [orb1239254]
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50 μl, 100 μlHuman B-Cell Activating Factor (BAFF/CD257) ELISA Kit [orb776009]
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To detect hBAFF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human BAFF (orb1272594) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBAFF.

To detect hBAFF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBAFF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
TNFSF13B Antibody (orb1272595)
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