BAFF Antibody

SKU: orb1239254

Description

BAFF Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-BAFF antibody (orb1239254) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF. The immunogen is located within the last 50 amino acids of BAFF.
• Target: TNFSF13B
• Molecular Weight: Predicted: 31kDObserved: 35 kD
• Purification: BAFF Antibody is Ion exchange chromatography purified.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: BAFF Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

BAFF Antibody: DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator

Western Blot Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M). Loading: 15 µg of lysates per lane. Antibodies: BAFF orb1239254 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: BAFF orb1239254 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human BAFF recombinant protein per lane. Antibodies: BAFF orb1239254 (Lane 1: 0.25 µg/mL; Lane 2: 0.5 µg/mL and Lane 3: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18kD.

Immunocytochemistry Validation of BAFF in HL60 Cells. Immunocytochemical analysis of HL60 cells using anti-BAFF antibody (orb1239254) at 1 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of BAFF in Human Spleen Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF with orb1239254 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Regulated Expression Validation of BAFF in Myeloma Patients (Tai et al., 2006). Immunoblot analysis was performed to monitor protein expression of BAFF with anti-BAFF antibodies in multiple myeloma cells with or without BMSCs. BAFF expression in cocultures at 8hr or 24hr was up-regulated by ~3.5-fold relative to BMSCs alone.

Immunohistochemistry Validation of BAFF in Thyroid of Patients with Graves' Diseases (Campi et al., 2015). BAFF expression detected by anti-BAFF antibodies (orb1239254) was remarkably increased in thyrocytes from multinodular goiter (C) compared with either Hashimoto's thyroiditis (E) or Graves' disease (G) while no staining was found in normal thyroid tissue (A).

Immunohistochemistry Validation of BAFF in Murine Cardiac Transplants at Rejection (Ye et al., 2004). BAFF expression detected by anti-BAFF antibodies (orb1239254) was upregulated in intragraft leukocytes due to rejection at 7 days after heart transplant.

Quick Database Links

Gene Symbol

TNFSF13B

UniProt

Q9Y275

RefSeq (Protein):NP_006564