TNFSF13B Rabbit Polyclonal Antibody

• Catalog Number: orb13273
• Category: Antibodies
• Description: TNFSF13B Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Predicted Reactivity: Bovine, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse TNFSF13B (221-309/309aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=3μg/Test, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: TNFSF13B
• UniProt ID: Q9WU72
• RRID: AB_10767956
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Tumor necrosis factor ligand superfamily member 13
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue): Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-BAFF antibody (orb13273), Dilution: 3 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Measured by its binding ability in a indirect ELISA. Immobilized TNFSF13B Peptides at 2 µg/mL (100 µl/well) can bind Rabbit Anti-TNFSF13B Antibody, the EC50 is 797.5 ng/ml.

Sample: Lane 1: Recombinant human TNFSF13B protein, N-His, Primary: Anti-TNFSF13B (orb13273) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kDa, Observed band size: 52 kDa.