ATG2A Antibody

SKU: orb699466

Description

Rabbit Anti-Human ATG2A Polyclonal

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Neuroscience

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution Range: WB (1:1000); ICC/IF (1:100)
• Reactivity: Human, Mouse
• Application Notes:
  A 1:1000 dilution was sufficient for detection of ATG2A on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminal of human ATG2A
• Target: ATG2A
• Molecular Weight: 90-100kDa
• Purification: Peptide Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATG2A, KIAA0404, ATG2 autophagy related homolog A, ATG2a_HUMAN, BC023754, 1810013C15Rik

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG2A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG2A Antibody (D) Composite.

Western blot analysis of Mouse Brain showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.

Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.

Quick Database Links

Gene Symbol

ATG2A

UniProt

Q2TAZ0

NCBI Gene ID

23130

RefSeq (Protein):NP_055919.2