You have no items in your shopping cart.
Cart summary
Item 1 of 3
Item 1 of 3
ATG2A Antibody: PerCP
Catalog Number: orb377373
| Catalog Number | orb377373 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody against ATG2A conjugated to PerCP. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, ICC, IF, IHC, WB |
| Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide from the C-terminal of human ATG2A |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000); ICC/IF (1:100) |
| Conjugation | PerCP |
| MW | 90-100kDa |
| Target | ATG2A |
| Entrez | 23130 |
| UniProt ID | Q2TAZ0 |
| NCBI | NP_055919.2 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | ATG2 autophagy related homolog A antibody, ATG2a_H Read more... |
| Note | For research use only |
| Application notes | A 1:1000 dilution of SPC-652 was sufficient for detection of ATG2A on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG2A Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG2A Antibody (D) Composite.
Western blot analysis of Mouse Brain showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 μg). Load: 20 μg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
