ATG16L Antibody

SKU: orb1933393

Description

ATG16L Antibody

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Dilution range: IF - 1:100, WB - 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 68265 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

APG16L

Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 μM, 16 h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ATG16L primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1 h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Western blot analysis of anti-APG16 Pab in Hela cell line lysates (35 ug/lane).APG16 (arrow) was detected using the purified Pab.

Quick Database Links

UniProt

Q676U5