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ATG16L1/ATG16L Antibody
Catalog Number: orb1537867
| Catalog Number | orb1537867 |
|---|---|
| Category | Antibodies |
| Description | ATG16L1/ATG16L Antibody |
| Target | ATG16L1 / ATG16L |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide |
| Purification | Peptide Column Affinity Chromatography |
| Immunogen | A 18 AA synthetic peptide near the center of human ATG16. The immunogen is located within aa 190-240 of ATG16. |
| Tested applications | ELISA, ICC, IF, IHC, IHC-P, WB |
| Dilution range | ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.25 - 0.5 µg/ml) |
| Application notes | Further information: ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.25 - 0.5 ug/mL. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 4 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | ATG16L1, APG16-like 1, APG16L, APG16L beta, ATG16L Read more... |
| Note | For research use only |
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of Anti-ATG16L1 antibody (orb1537867, 1 µg/ml; 15 µg of lysate per lane). Lane 1: A431 cell line. Lane 2: A549 cell line. Lane 3: Caco2 cell line. Antibody produced bands at ~68 kDa (Isoform 1), and ~66 kDa (isoform 2)
Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 ug/ml.
Immunofluorescence of ATG16 in HeLa cells with ATG16 antibody at 4 ug/ml.
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