ATG16L1/ATG16L Antibody

SKU: orb1537867

Description

ATG16L1/ATG16L Antibody

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, IHC-P, WB
• Dilution range: ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.25 - 0.5 µg/ml)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Further information: ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.25 - 0.5 ug/mL. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 4 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A 18 AA synthetic peptide near the center of human ATG16. The immunogen is located within aa 190-240 of ATG16.
• Target: ATG16L1 / ATG16L
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

ATG16L1, APG16-like 1, APG16L, APG16L beta, ATG16L, Autophagy-related protein 16-1, IBD10, WD repeat domain 30, ATG16A, WDR30

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot analysis of Anti-ATG16L1 antibody (orb1537867, 1 µg/ml; 15 µg of lysate per lane). Lane 1: A431 cell line. Lane 2: A549 cell line. Lane 3: Caco2 cell line. Antibody produced bands at ~68 kDa (Isoform 1), and ~66 kDa (isoform 2)

Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 ug/ml.

Immunofluorescence of ATG16 in HeLa cells with ATG16 antibody at 4 ug/ml.

Quick Database Links

Gene Symbol

ATG16L1 / ATG16L