ATG16L Antibody

SKU: orb33384

Description

Purified rabbit polyclonal antibody.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IF/ICC: 1:25, IHC-P: 1:10-50
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD29612
• Immunogen: Synthetic Peptide
• Target: ATG16L1
• Molecular Weight: 68265
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-hCG_1817841 antibody, anti-APG16L antibody, anti-ATG16L antibody, anti-FLJ00045 antibody, anti-FLJ10035 antibody, anti-FLJ10828 antibody, anti-FLJ22677 antibody, anti-IBD10 antibody, anti-WDR30 antibody, anti-APG16 autophagy 16-like antibody, anti-APG16-like 1 antibody, anti-APG16L beta antibody, anti-ATG16 autophagy related 16-like protein 1 antibody, anti-WD repeat domain 30 antibody, anti-autophagy-related protein 16-1 antibody

Western blot of APG16L (L92) Pab in mouse brain tissue lysate.

Cos7, HEK293, MEF, and Hela cells, left to right, respectively.

APG16L Antibody western blot analysis in NCI-H460 cell line lysates (35 ug/lane). This demonstrates the APG16L antibody detected the APG16L protein (arrow).

Anti-ATG16L Antibody at 1:1000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of U251 cells, using ATG16L Antibody. U251 cells (right) were treated with Chloroquine (50 μM, 16 h). Diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with Autophagy APG16L antibody (L176), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

ATG16L1

UniProt

Q676U5

RefSeq (Protein):NP_110430.5, NP_942593.2, NP_001177195.1, NP_001177196.1, NP_060444.3

Zhang, Zhengang et al. Intraflagellar transport protein IFT20 is essential for male fertility and spermiogenesis in mice Mol Biol Cell, (2016)

PubMed: 27682589