ASPN Antibody

SKU: orb1248974

Description

ASPN Antibody

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in Human Tonsil and Uterus and in Mouse and Rat Skeletal Muscle lysates (calculated MW of 43.3kDa according to Human NP_060150.4, and 42.6kDa acciording to Mouse NP_001165952.1 and Rat NP_001014030.1). Recommended concentration: 0.1-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Cerebellum lysate.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-IHENKVKKIQKDT
• Target: ASPN
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

ASPN, PLAP1, SLRR1C, FLJ20129, asporin (LRR class 1), small leucine-rich protein 1C, periodontal ligament associated protein 1, asporin, OS3, asporin proteoglycan

orb1248974 (0.1 ug/ml) staining of Human Tonsil (A), (0.3 ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1 ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35 ug protein in RIPA buffer).Detected by chemiluminescence.

orb1248974 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear membrane staining.

Quick Database Links

Gene Symbol

ASPN

UniProt

Q9BXN1

RefSeq (Protein):NP_060150.4