Goat anti-Asporin / ASPN Antibody

SKU: orb18981

Description

Goat polyclonal antibody to ASPN

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 40kDa band observed in Human Bone Marrow lysates (calculated MW of 43.4kDa according to NP_060150.3). Recommended concentration: 1-3 μg/ml.Experiment Notes: Immunofluoresence: in cell line ATDC5 shows signals in cytoplasm and on the cell surface. Data gratefuly received from Dr. Shiro Ikegawa, SNP Research Center, RIKEN, Japan.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Asporin / ASPN
• Protein Sequence: IHENKVKKIQKDT
• Molecular Weight: 43.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ASPN antibody, anti PLAP1 antibody, anti SLRR1C antibody, anti FLJ20129 antibody, anti asporin (LRR class 1) antibody, anti small leucine-rich protein 1C antibody, anti periodontal ligament associated protein 1 antibody, anti asporin antibody, anti OS3 antibody, anti asporin proteoglycan antibody

Immunofluorescence analysis of HeLa cells using ASPN antibody

Western blot analysis of human bone marrow lysate using ASPN antibody

Confocal immunofluoroscence analysis of ATDC5 cells using ASPN antibody

Western blot analysis of Human Tonsil(lane 1), Human Uterus(lane 2), Mouse Skeletal Muscle(lane 3), Rat Skeletal Muscle(lane 4) and Human Cerebellum(lane 5) lysate using ASPN antibody

Quick Database Links

Gene Symbol

Asporin / ASPN

NCBI Gene ID

54829