ARHGEF4 Antibody

• Catalog Number: orb1247774
• Category: Antibodies
• Description: ARHGEF4 Antibody
• Target: ARHGEF4
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: PWEEPAGEKPSCS
• UniProt ID: Q9NR80
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 127462.1). Recommended concentration: 2-4ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ARHGEF4, Rho guanine nucleotide exchange factor (G
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_056135.2, NP_127462.1

orb1247774 (2 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ARHGEF4 and probed with orb1247774 (mock transfection in first lane).