Goat anti-ARHGEF4 Antibody

SKU: orb18934

Description

Goat polyclonal antibody to ARHGEF4

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 2-4 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 127462.1). Recommended concentration: 2-4 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ARHGEF4
• Protein Sequence: PWEEPAGEKPSCS
• Molecular Weight: 79.1; 76.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ARHGEF4 antibody, anti Rho guanine nucleotide exchange factor (GEF) 4 antibody, anti ASEF antibody, anti GEF4 antibody, anti STM6 antibody, anti KIAA1112 antibody, anti DKFZp434G2016 antibody, anti APC-stimulated guanine nucleotide exchange factor antibody, anti ASEF1 antibody, anti Rho guanine nucleotide exchange factor 4 antibody

orb18934 (2ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ARHGEF4 (RC215591) and probed with orb18934 overexpressing ARHGEF4 (RC215591) and probed with orb18934 (mock transfection in first lane), tested by Origene. (mock transfection in first lane), tested by Origene.

Quick Database Links

Gene Symbol

ARHGEF4

NCBI Gene ID

50649