ARHGEF4 Antibody

SKU: orb154413

Description

Mouse monoclonal antibody to ARHGEF4 which acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. It binds to APC may starts RAC1 GEF activity.

Images & Validation

• Tested Applications: FC, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: ARHGEF-08
• Immunogen: Recombinant fragment of human ARHGEF4 (amino acids 143-271)
• Target: ARHGEF4
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASEF1, GEF4, STM6

Western blotting analysis of ARHGEF4 in HEK293 cells (A) and HEK293-ARHGEF4 transfectants (B) using mouse monoclonal anti-ARHGEF4 (clone ARHGEF-08).

Separation of cells stained using anti-ARHGEF4 (ARHGEF-08) purified antibody (concentration in sample 4 µg/ml, GAM APC, red-filled) from cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining) of ARHGEF4 transfected HEK-293 cell suspension.

Quick Database Links

Gene Symbol

ARHGEF4

UniProt

Q9NR80

NCBI Gene ID

50649