APAF1 Rabbit Polyclonal Antibody

SKU: orb624763

Description

Rabbit polyclonal antibody to APAF1

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, IP, WB
• Dilution Range: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:20-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: apoptotic peptidase activating factor 1
• Target: APAF1
• Molecular Weight: 130-142 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KIAA0413

Immunofluorescent analysis of A549 cells using orb624763 (APAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-APAF1 (IP: orb624763, 4 ug; Detection: orb624763 1:500) with HEK-293 cells lysate 2000 ug.

HEK-293 cells were subjected to SDS PAGE followed by western blot with orb624763 (APAF1 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human heart tissue slide using orb624763 (APAF1 Antibody) at dilution of 1:200.

Quick Database Links

Gene Symbol

APAF1

UniProt

O14727