APAF1 Antibody

• Catalog Number: orb624763
• Category: Antibodies
• Description: Rabbit polyclonal antibody to APAF1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: apoptotic peptidase activating factor 1
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:20-1:200
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 130-142 kDa
• Target: APAF1
• UniProt ID: O14727
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: KIAA0413
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of A549 cells using orb624763 (APAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-APAF1 (IP: orb624763, 4 ug; Detection: orb624763 1:500) with HEK-293 cells lysate 2000 ug.

HEK-293 cells were subjected to SDS PAGE followed by western blot with orb624763 (APAF1 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human heart tissue slide using orb624763 (APAF1 Antibody) at dilution of 1:200.