NLRP3 Antibody

• Catalog Number: orb665921
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cryopyrin
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant protein of human NLRP3. The exact sequence is proprietary.
• UniProt ID: Q96P20, Q8R4B8
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1-500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C1orf7; CIAS1; NALP3; PYPAF1; NACHT, LRR and PYD d
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 216799, 114548
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NLRP3 expression in HT29 (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 83; 105; 111; 112; 115; 118 kD; Observed band size: 118 kD)

Immunohistochemical analysis of NLRP3 staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of NLRP3 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.